mouse podocyte cell line  (Procell Inc)

Journal: PeerJ

Article Title: The cuproptosis-related gene ITGB6 and LTBP1 may be associated with diabetic kidney disease progression and immune cell infiltration

doi: 10.7717/peerj.20346

Figure Lengend Snippet: MPC5 cells were treated with high glucose (30 mM) for 24, 48, and 72 h. (A) LTBP1 expression increased significantly compared to control ( P < 0.05) at 48 h. Data are shown as mean ± SEM, n = 3. * P < 0.05. (B) ITGB6 expression increased significantly compared to control ( P < 0.05) at 24, 48 and 72 h. Data are shown as mean ± SEM, n = 3. * P < 0.05; ** P < 0.01, *** P < 0.001.

Article Snippet: (1) Cell culture : The mouse renal podocyte cell line MPC5 was obtained from Procell Life Science & Technology Co., Ltd. (catalog number: CL-0855; RRID: CVCL_0496).

Techniques: Expressing, Control

Journal: eLife

Article Title: Empagliflozin reduces podocyte lipotoxicity in experimental Alport syndrome

doi: 10.7554/eLife.83353

Figure Lengend Snippet: ( A–D ) Immortalized podocytes and tubular cells of wild-type (WT) and AS mice treated with empagliflozin (Empa) or vehicle for 48 hr. ( A,C ) Bar graph analysis showing cytotoxicity normalized to viability, then compared to WT (n=3). ( B,D ) Bar graph analysis showing apoptosis normalized to viability, then compared to WT (n=3). ( E,F ) LD accumulation in tubular cells ( E ) and podocytes ( F ) was measured by Nile red staining. Bar graph analysis showing the quantification of the number of LDs per cell (n=3). ( G ) Correlation analyses between the LD accumulation and apoptosis in podocytes (n=12). ( H ) Representative images of Nile red staining demonstrate increased LD numbers (Nile red: green) in AS podocytes (Cell mask blue: blue; DAPI: red) compared to WT podocytes, which is reduced by Empa treatment. ( A–F ), Two-tailed Student’s t-test. ( G ), Pearson’s correlation coefficient. *p<0.5, **p<0.01.

Article Snippet: To establish immortalized mouse podocyte and tubular cell lines, Col4a3 +/- mice were bred with the immorto-mice carrying a temperature-sensitive T-antigen transgene (SV40 + ) (Charles River, Wilmington, MA, CBA/CaxC57BL/10-H-2Kb-tsA58) ( ) to generate double heterozygous littermates, which were then crossed to generate SV40 + ; Col4a3 -/- (immorto-AS) and SV40 + ; Col4a3 +/+ (immorto-WT) ( ; ).

Techniques: Staining, Two Tailed Test